Protein Analysis is one of the protein methods used to study protein. Protein Analysis can be also called as protein characterization. Why it can that important to study protein? Proteins are polymers of up to 20 different types of amino acids and exist in different forms like peptide, polypeptide, enzyme, hormone or protein molecules besides the amino acids mentioned. Proteins differ from each other by their type, number and sequence of amino acids that make up the polypeptide backbone. Different proteins have different molecular structures, nutritional attributes and physiochemical properties. Protein is indicator of biological activity and entity. Protein analysis together with quantitative proteomics are used to determine the quality and quantity of protein to let us understand the biological reaction or process.
To identify the amount of proteins in a sample or to differentiate the analysis in sample, Quantitative Proteomics is a key analytical chemistry technique. The including quantification for protein identification works as an additional dimension to the general, qualitative proteomics analysis. Compared to just providing the list of proteins in a sample, quantitative proteomics can also give the information to identify the physiological differences between samples which can be used to study the differences between the diseased and normal population, before and after medication, the impact of the antibiotic to biofilm, etc.